Cortisol (Stress Hormone) Rapid Saliva Test

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Cortisol (Stress Hormone) Rapid Saliva Test

Cortisol (Stress Hormone) Rapid Saliva Test

Screen for elevated cortisol using a saliva sample. Cortisol helps the body respond to stress, and levels naturally change throughout the day.

This test shows whether cortisol in saliva is above the cut-off of 50 ng/mL.

Quick results in 3 minutes. No lab send-off required.

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About this test

Cortisol is a hormone made by the adrenal glands. It’s often called the stress hormone because it's an important part of the body's response to stress. Your cortisol levels naturally rise and fall throughout the day. That means the time you administer a saliva sample matters when interpreting the result.

This rapid saliva test is for professional use. The result shows if cortisol in the sample is above the test’s cut-off. A healthcare professional can interpret it in context and decide if further testing is needed.

Symptoms of chronically high cortisol can include the following:

  • Weight gain, particularly around the chest and tummy, with thinner arms and legs
  • Skin that bruises easily or develops wide, purple stretch marks
  • Muscle weakness, especially in the upper arms or thighs
  • The face becoming rounder or redder over time

These changes can have several causes, so cortisol should only be part of the investigation.

Read more about what high cortisol levels mean.

What this test offers

Simple saliva collection
97.3% accuracy
98.7% specificity
Cut-off at 50 ng/mL
First reading at 3 minutes
No sample send-off required
Learn more about this test

Frequently Asked Questions

What does this saliva cortisol test tell you?

The test checks if there’s more than 50 ng/mL of cortisol in the saliva sample. If there is, the result is positive. The kit doesn’t tell you how far above the cut-off the sample was. 

If you’re having the test because you’ve been feeling unwell, the professional reading it can look at the result alongside your symptoms and decide if it’s worth doing any further tests.

How is this rapid test different from a lab-based saliva cortisol test?

If your GP has asked for a saliva sample at bedtime, use the kit and collection time they give you. They may be checking if your cortisol falls at night as expected. The lab measures how much cortisol is in the sample, and your clinician compares that number with the range they’d expect at that hour.

With Rezure’s rapid test, the professional who collects the sample can read the result there and then, without sending it away. They get a positive or negative result based on the kit’s cut-off. 

What should I avoid before taking a saliva sample for the cortisol test?

If you’ve just had a drink, even water, wait at least 30 minutes before giving your sample. The same goes for food, chewing gum and tobacco. 

Tell the person taking the sample if your gums are bleeding, as blood in the saliva can interfere with the test.

How does the time of day affect cortisol levels?

Say one sample was taken at 8 am and the other at 8 pm. If you usually sleep at night, you’d expect cortisol to be higher in the morning, around the time you wake, and lower by the evening. 

That can happen on an ordinary day when you feel much the same from breakfast to bedtime. If samples are being collected on different days for comparison, make a note of the time each one was taken and aim for the same hour where practical. 

Learn more on our blog: When is the best time to test cortisol?

Can a negative result rule out a cortisol problem?

No. The kit has one cut-off, 50 ng/mL, and a negative result means the saliva sample came in below it. Cortisol changes over the course of a day, so the time the sample was taken is part of the picture.

If you had the test because something’s been bothering you and it’s still happening, speak to your GP. Tell them what prompted the test and bring the result, along with the time of collection if you have it.

What do 97.3% accuracy and 98.7% specificity mean in practice?

Specificity is easiest to picture if you start with samples that should all come back negative. At 98.7% specificity, the kit would correctly call about 987 out of 1,000 of those samples negative. The other 13 would come back positive by mistake.

The 97.3% accuracy figure covers the whole set of samples in the manufacturer’s evaluation. It counts the positives the kit got right as well as the negatives.

Can I use saliva collected separately, or do I need to collect it with the supplied device?

If you’ve already collected saliva in a cup, start again with a fresh sample. The instructions for this kit have you collect saliva straight onto the absorbent tip under the tongue. 

The collector is part of the test itself, so you use the same device from collection through to the result.

Does the kit come with everything I need to collect the sample?

Yes. The pack contains the test device with its cap and the instruction leaflet. The collection tip is already fitted, so you can leave the swabs and sample pots in the cupboard. 

The one thing to have handy is a timer for reading the result.

Can I order this test in bulk for a clinical practice?

Yes. To get a quote, tell us roughly how many cortisol tests you need and when you need them. If you expect to order regularly, give us an idea of how many you’d use in a typical month. 

We can quote for a one-off order or discuss a repeat supply. Ask about bulk pricing

Can medicine affect the result of a cortisol test?

Yes. Steroid medicines are worth flagging before you give a saliva sample, and that includes inhalers and creams as well as tablets. 

Tell the person carrying out the test what you use and when you last took or applied it. Keep taking prescribed medicine as usual unless the person who prescribed it advises you to change how you take it.

What should happen after a positive result?

Speak to the healthcare professional who arranged the test. If you’re taking the result to your GP, tell them why you had the test and, if you know, when the sample was taken.

The result means the cortisol in that sample was above the kit’s 50 ng/mL cut-off. The next step depends on what prompted the test in the first place. Your GP may ask about your symptoms and medicines, then decide if a blood test, a urine test or a timed saliva sample would help.

How to use this test

1
Prepare the test

Allow the testing device to reach room temperature (15-30°C) before starting.

Ensure the donor has not consumed food, drink, chewing gum, or tobacco products for at least 30 minutes before collection.

2
Collect the saliva sample

Remove the protective cap from the testing device. 

Ensure the mouth has ample oral fluid pooled for the sample, for at least 30 seconds.

Place the absorbent sponge under the tongue for 1 minute until fully saturated.

Then, swab the gums around your mouth 10-15 times to help with absorption.

3
Observe the flow and time results

Keep the device inserted in the mouth until the flow appears on the cassette (around 1 minute). Once the flow begins, remove the device, place it on a flat, clean surface, and start timing.

4
Reading the results and invalid samples

Results must be read between 3 and 10 minutes. 

INVALID: No line appears in the control region (C). This may result from insufficient sample or procedural errors. Repeat the test with a new cassette. If the issue continues, discontinue use and contact your local distributor.

5
Reading a negative result

NEGATIVE: Two colored lines appear—one in the control region (C) and one in the test region (T). This indicates cortisol is below the detectable level. Even a faint line in the test region should be considered negative.

6
Reading a positive result

POSITIVE: Only one colored line appears in the control region (C), with no line in the test region (T). This indicates that cortisol exceeds the detectable level.

  • Instant, clear results

    Get an immediate answer at home in 10 minutes. No waiting around.

  • CE-certified & regulated

    Meets European standards for safety and reliability.

  • Clinically validated

    Tested to ensure accurate and consistent results.

  • Exceptional accuracy

    Detects even small traces clearly and reliably, every time.

Cortisol testing for your practice

Collect a saliva sample and read the result in your practice. There’s no sample to send away.

Order a single test or choose a pack of 2 or 5. If you need a larger quantity or expect to order regularly, tell us how many tests you’ll need and we’ll put together a quote.

Ask about bulk orders